Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology The role of oxidative stress, inflammation and acetaminophen exposure from birth to early childhood in the induction of autism William Parker, Chi Dang Hornik, Staci Bilbo, Zoie E. Holzknecht, Lauren Gentry, Here's how to make Tylenol work way better Dose it correctly: 1,000 mg at a time, spaced every 8 hours. Smaller doses don't control pain as well. Take it