Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen metabolism Tylenol Glutathione Kizer Wellness LLC The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link
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glutathione after tylenol Acetaminophen-Induced Hepatotoxicity: a Comprehensive Update