Replying to @Angela Vitamin B12 supplements can trigger an acne like eruption, as can vitamin B6 & biotin supplements. In contrast to acne, there are no comedones. Once the supplements are stopped, What should I do if I experience a breakout after taking a vitamin B12 supplement? Did Vitamin B12 Give Me Acne? Mixed Makeup Is B12 making you break out? It's possible. We've found in our own experience and in clinical studies that certain forms of vitamin B12 can trigger inflammatory acne for some individuals.