Tylenol: How much is too much of a good thing? Allied Medical Training Efficacy of Oral N Acetylcysteine in the Treatment of Acetaminophen Overdose New England Journal of Medicine Acetaminophen Pathway (therapeutic doses), Pharmacokinetics Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.